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Image Search Results
Journal: Frontiers in Microbiology
Article Title: DNA Engineering and Hepatitis B Virus Replication
doi: 10.3389/fmicb.2021.783040
Figure Lengend Snippet: Identification of two regions suitable for engineering. (A) Sequences that were deleted in different constructs. Deletion variants were named according to the sequences deleted. The black cycle and black triangle represent the start codon and the stop codon, respectively. The hybridization probe is shown (1199–1814). (B) The mutations of pCH9-Δε. Sequence from 1858 to 1863 was deleted, and two additional mutations, G1877T and T1878A, were introduced. (C) Representative Southern blotting of the deletion variants. The plus sign (+) denotes Eco RI digestion. RC, DL, SS DNA, and Eco RI digested fragments are indicated, respectively. (D) The relative intensity of RC DNA of deletion variants. The intensity of RC DNA of each sample was divided into the sum of RC DNA of all samples in the same membrane, respectively, to calculate the relative amount of RC DNA. N = 5. Asterisks (*) denote the difference between a deletion variant and pCH9-G2016T is statistically significant ( p < 0.05). (E) Normal QQ plot of the relative amount of the RC DNAs.
Article Snippet:
Techniques: Construct, Hybridization, Sequencing, Southern Blot, Membrane, Variant Assay
Journal: Frontiers in Microbiology
Article Title: DNA Engineering and Hepatitis B Virus Replication
doi: 10.3389/fmicb.2021.783040
Figure Lengend Snippet: RNA assay of the deletion variants. (A) The sequence of HBV transcripts. The wave lines represent RNA; the black rectangles represent 5′ cap; the multiple arrowheads represent the poly-A tail. The black cycles and black triangles represent the start codon and the stop codon, respectively. The length of each transcript is an estimation without a poly-A tail. (B) Representative Northern blotting results of the deletion variants. (C) The relative intensity of pgRNA of the deletion variants. The intensity of each pgRNA/18s rRNA was divided into the sum of pgRNA/18s rRNA of all samples in the same membrane, respectively, to calculate the relative intensity of pgRNA of each sample. N = 4. Asterisks (*) denote the difference between deletion variants and pCH9-G2016T is significant ( p < 0.05). (D) Normal QQ plot of the relative amount of the pgRNAs.
Article Snippet:
Techniques: Sequencing, Northern Blot, Membrane